loading page

Comparisons of Whole Saliva and Cell Free Saliva by DIA-Based Proteome Profiling
  • +9
  • Lingling Jiao,
  • huilin Dong,
  • yanhua Qin,
  • jun Zhu,
  • penglin Wu,
  • jing Liu,
  • yi Cao,
  • changjian Wu,
  • yuan Zhang,
  • fan Cao,
  • feng Li,
  • huaiyuan Zhu
Lingling Jiao
China Tobacco Jiangsu Industrial Co Ltd
Author Profile
huilin Dong
China Tobacco Jiangsu Industrial Co Ltd
Author Profile
yanhua Qin
China Tobacco Jiangsu Industrial Co Ltd
Author Profile
jun Zhu
China Pharmaceutical University School of Traditional Chinese Pharmacy
Author Profile
penglin Wu
China Tobacco Jiangsu Industrial Co Ltd
Author Profile
jing Liu
China Tobacco Jiangsu Industrial Co Ltd
Author Profile
yi Cao
China Tobacco Jiangsu Industrial Co Ltd
Author Profile
changjian Wu
China Tobacco Jiangsu Industrial Co Ltd
Author Profile
yuan Zhang
China Tobacco Jiangsu Industrial Co Ltd
Author Profile
fan Cao
China Tobacco Jiangsu Industrial Co Ltd
Author Profile
feng Li
China Tobacco Guangdong Industrial Co., Ltd
Author Profile
huaiyuan Zhu
China Tobacco Jiangsu Industrial Co Ltd

Corresponding Author:[email protected]

Author Profile

Abstract

Saliva has emerged as a promising diagnostic resource due to its accessibility, non-invasiveness, and repeatability, enabling early disease detection and timely intervention. However, current studies often overlook the distinction between whole saliva (WS) and cell-free saliva (CFS). This study aims to compare the proteomic profiles of WS and CFS, both with and without low-abundance proteins enrichment using nanoparticles, employing DIA-MS technology. Our findings reveal a substantial enhancement in the detectability of low-abundance proteins in saliva with utilization of nanoparticles, enabling identification of 12%-15% low-abundance proteins previously undetectable in WS or CFS. In total, 3817 saliva proteins were identified, with 3413 found in WS and 2340 in CFS. More interestingly, we found that it wasn’t the similarity of the samples that did the clustering, but rather it depended more on the different detection methods and sample types. And the predominant functions of the identified proteins in WS were related to oxidative phosphorylation and neurodegenerations, whereas those in CFS were primarily associated with nitrogen and glycosaminoglycan metabolism. And both exhibited functions in immune response and proteasome. This study represents the first comparison of WS and CFS, providing valuable experimental evidence for guiding the selection of research subjects in future salivomics studies.